Samantha Louise "Lou" Fleming-Bartlet-Morris is Amy's older sister. At the beginning of the series she moves back home from her job in New York to help get Heartland back running again after her mother's death. She first has a New York boyfriend (Carl). She later gets together with the veterinarian Scott Cardinal. Eventually she meets her husband, Peter, thanks to Lisa. Their relationship is secret at first, because Lou knows Jack won't approve to him due to his job in the oil industry. The couple marry at the end of season 3. They move to Dubai, but Lou soon feels very alone. She grows to miss Heartland and her family. When Lou and Peter move back home, Lou finds out she is pregnant with her daughter, Catherine. She later gives birth to a baby girl, whose name is Catherine Marion Minnie "Katie" Fleming-Morris. Amy Fleming and Ty Borden deliver the baby. She later adopts a foster child named Georgina "Georgie" Crawley.
Dealing with the loss of her mother after she died in a car wreck, Lou moves back to Heartland from New York to help bring the ranch out of financial troubles. Lou also begins dating the ranch veterinarian, Scott Cardinal.
Lou and Peter officially adopt Georgie. Maggie's Diner is up for sale. Lou fights to have Maggie's Diner turned into a heritage site to keep it from becoming a chain food store. After it's declared a heritage site, she purchases it with the help of her father, Tim. An old friend of Lou's becomes the manager of the diner.
This year finds Lou juggling her responsibilities at Heartland and as an entrepreneur while facing the challenges of single motherhood. Initially Lou overcompensates, hoping to diminish the separation’s impact on her girls. But she clashes with her rebellious teenage daughter, leading to a disturbing call from Georgie’s school that threatens to tear the family apart. As Lou takes tentative steps to explore what life after marriage might look like, she dips her toe back into the dating pool with mixed results. The end of the Season sees Lou lauded for her professional accomplishments, while her personal life is again in flux. When forced to make a tough decision about her family’s future, Lou realizes she needs to move on.
Dr. Scott Cardinal - The two rekindle their romance after Lou returns from New York. They break up in season 2.
Constable Mark Rodriguez - Lou began a relationship with Mark after he brings his horse to Heartland to have Amy work with him to get over his fear of water. Just as Lou was starting to open her heart to Mark she ran into him in town kissing another girl.
Peter Morris - After Lisa gave Lou's email address to a friend of her friend who passed it on to her son unknowingly, the son, Peter contacted Lou on a whim of fate. Lou eventually replied to try online dating and start getting over her experiences with both Scott & Mark. They scheduled their first meet up date before Lou realized she had seen him in person before. He is known as the oil man who she went head to head with when his company Bedford Oil was drilling on Jack's property. After she found out who he was, she wasn't interested in dating him, but he pushed her to give it a try. They kept it secret initially. They eventually married and have two daughters, Katie and Georgie.